Detalle del congreso

Autores: Krsticevic, Flavia; Emmel Vanessa; Ezpeleta, Joaquín; Murillo, Javier; Gama Bianca; Bulacio, Pilar; Vera-Lozada, Gabriela; Ponce, Sergio; Abdelhay Eliana; Tapia, Elizabeth; Hassan, Rocio.

Resumen: The portable MinION sequencer (ONT) has become mainstream due to its small size and cost-effective device. Now, it is an essential tool for pathogen surveillance and clinical diagnostic as these applications would benefit from the real-time nature of this technology. Despite all the advantages, ONP reads bear high rates of errors (>15%). Barcodes are not resistant to the high sequencing errors which interfere negatively in the correct sequence determination, a critical issue in clinical diagnostics or sample characterization. The objective of this work was to develop a method for multiplex ONP sequencing. We took advantage of MinION to perform a rapid characterization of the E1 Chikungunya virus gene by multiplexing a high number of clinical samples from cancer individuals infected with the virus.

Tipo de reunión: Simposio.

Tipo de trabajo: Resumen.

Producción: AN EFFECTIVE BARCODING OF VIRAL CLINICAL SAMPLES LIBRARIES FOR MINION (ONP) MULTIPLEX SEQUENCING.

Reunión científica: XII Simpósio de Oncobiologia.

Lugar: Rio de Janeiro.

Institución organizadora: Universidade Federal do Rio de Janeiro.

Publicado: Sí

Lugar publicación: Rio de Janeiro

Mes de reunión: 9

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