Authors: Flavia Krsticevic; Melissah Rowe; Ivaneide Furtado; Javier Murillo; Joaquín Ezpeleta; Sergio Ponce; Elizabeth Tapia; Marlucia Martins.
Resumen: Severalstudies reported low-diversity of bacteria associated with naturalpopulations of Drosophila from different habitats and distantgeographic locations. In populations of Neotropical Drosophilidae,microbiomes are poorly characterized. The primary forest shifts dueto agricultural activities and it is not well established how theflies deal with these changes. The microbiome can affect the fitnessof adults and therefore their resilience to environmental stress. Theaim of our study was to determine the diversity of the bacterialcommunities associated with Drosophilidae species in primary forestand disturbed environments of the state of Para, Brazil. Flies werecollected from their natural feeding substrates -flowers andmushrooms- in Belém and Tailándia and attracted with artificialbanana baits in samples of primary forest and disturbed environmentsin Tailândia. DNA was extracted from samples of Hirtodrosophila sp.,a mushroom-feeder, D. speciosa, a flower-feeder, and D. sturtevanti,a fruit-feeder. Multiplexed samples with the V3-V4 regions of 16SrRNA amplicons were run on an Illumina Hiseq-2000 platform. Theexperiment was controlled using almost three biological replicatesand one technical triplicate, a mock community, and a negativecontrol. The reads generated were demultiplexed with Mothur andclassified using Centrifuge program in order to reduce a memoryusage. A total of 42 families and 142 species were classified. Infruit-feeding hosts, a low-diversity -6.2 families and 15.8 species-was found in samples from in primary forest. Contrary, samples fromdisturbed environments are highly diverse with 10.33 families and23.66 species. The flower -6 families and 12 species- and themushroom feeders -8 families and 20.2 species- tend to have a similardiversity values than flies from primary forest, while their specificcomposition is shaped by the host diet. The intracellular Wolbachiawas found in D.speciosasamples.
Meeting type: Simposio.
Production: Microbiota associated to natural populations of Drosophilidae in the Brazilian Amazon.
Scientific meeting: X Symposium on Ecology, Genetics and Evolution ​ ​ of ​ ​ Drosophila ​ ​ (X ​ ​ SEGED).
Meeting place: Ouro Preto.
It's published?: No
Meeting month: 11